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Enzymatic Complex Summary

Exosome from Homo sapiens

Enzyme type: 3'-5'-exoribonuclease


Structure of Exosome (Homo sapiens):

Enzymatic components:
 
Exosome core
 
Protein components:
 
Exosome component 10
Exosome complex exonuclease RRP44
DIS3-like exonuclease 1
 

There are two genes in the human genome that encode DIS3 proteins: hDIS3 and hDIS3L - they both interact andcooperate with the human core exosome. They have different intracellular localization patterns:

  • hDIS3 is mainly nuclear
  • hDIS3L is strictly cytoplasmic
Biochemical analyses indicate that both proteins are active 3' - 5' exonucleases but only hDIS3 displays endonucleolytic activity, which is in agreement with the preservation of the PIN active site in hDIS3 but not in hDIS3L. These results suggest that in humans the exosome complex endowed with endonucleolytic activity is mainly nuclear. [PMID: 20675404]


Reactions in which Exosome is involved:
Pathways in which Exosome is involved:
References:

Authors Title Journal
Lykke-Andersen S, Tomecki R, Jensen TH, Dziembowski A The eukaryotic RNA exosome: same scaffold but variable catalytic subunits. RNA Biol 2011-02-01
Tomecki R, Dziembowski A Novel endoribonucleases as central players in various pathways of eukaryotic RNA metabolism. RNA 2010-09-01
Schaeffer D, van Hoof A... Different nuclease requirements for exosome-mediated degradation of normal and nonstop mRNAs. Proc Natl Acad Sci U S A 2011-01-08
Schneider C, Leung E, Brown J, Tollervey D... The N-terminal PIN domain of the exosome subunit Rrp44 harbors endonuclease activity and tethers Rrp44 to the yeast core exosome. Nucleic Acids Res 2009-03-01



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Last modification of this entry: Sept. 17, 2012.

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