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Enzymatic Complex Summary

CFI from Homo sapiens

Known also as: Cleavage factor Im complex, CFIm


Structure of CFI (Homo sapiens):

 
Protein components:
Cleavage and polyadenylation specificity factor subunit 5
Cleavage and polyadenylation specificity factor subunit 6
Cleavage and polyadenylation specificity factor subunit 7
 

 

Pre-mRNA cleavage and polyadenylation site selection involves the cleavage/polyadenylation complex that consists of cleavage polyadenylation specificity factor (CPSF), cleavage factors Im and IIm (CFIm and CFIIm), cleavage stimulatory factor (CstF), poly (A) polymerase (PAP), and poly (A) binding protein II. 
 
CFIm facilitates assembly of the 3′ processing complex and enhances both the rate and efficiency of poly(A) site cleavage in vitro. This factor binds to pre-mRNA at a very early step and facilitates the binding of other 3′-end processing factors via direct interaction with and recruitment of CPSF, but not CstF or PAP. Multiple UGUAN sites flanking the hexamer site contribute to CFIm binding based on gel shift analysis. Further, these sites promote both pre-mRNA cleavage and poly(A) addition using in vitro reconstitution assays. CFIm is a heterodimer consisting of a 25-kDa protein (NUDT21/CPSF5) and a larger subunit. A complex consisting of NUDT21 and a 68-kDa subunit protein (CPSF6) or 59-kDa subunit protein (CPSF7) are sufficient for RNA binding, and this heterodimer reconstitutes CFIm processing activity. Additional larger subunit (72 kDa) also has been identified, but its significance remains uncertain.
 

 



Reactions in which CFI is involved:
Pathways in which CFI is involved:
References:

Authors Title Journal
Sartini BL, Wang H, Wang W, Millette CF, Kilpatrick DL Pre-messenger RNA cleavage factor I (CFIm): potential role in alternative polyadenylation during spermatogenesis. Biol Reprod 2008-03-01



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Last modification of this entry: Aug. 22, 2012.

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