Welcome! Click here to login or here to register.
Catalytic RNA molecules
Enzymatic complexes
Draw a picture

Bujnicki Lab Homepage

Enzymatic Complex Summary

C/D-box snoRNP from Saccharomyces cerevisiae

Structure of C/D-box snoRNP (Saccharomyces cerevisiae):

Protein components:
Nucleolar protein 56
rRNA 2'-O-methyltransferase fibrillarin
Nucleolar protein 58
13 kDa ribonucleoprotein-associated protein
RNA components:
C/D box snoRNA

Yeast rRNAs contain about 65 methyl groups; 55 of these are linked to the 29-hydroxyl residue of ribose, while the rest of them are attached to the bases. Ribose methylation is, in analogy to pseudouridylation, carried out by specific snoRNPs that contain snoRNAs of the C/D-box family. These snoRNAs have one or two sets of well-conserved short sequence elements, which are called box C and box D (or box C9 and box D9, for the second set), and about 10 to 20 nucleotides with sequence complementarity to the rRNA. The position of 29-O-ribose methylation of rRNA is 5 nucleotides upstream of box D (or D9). Most of the 55 29-O-ribose-methylated sites have been recently assigned to different guide C/D-box snoRNAs, all of them dispensable for cell viability. In contrast to these, U3 and U14 are essential C/D-box snoRNAs. The U3 snoRNA seems to be exclusively required for pre-rRNA processing at sites A0 to A2, while the U14 snoRNA is involved in pre-rRNA processing reactions at sites A1 and A2 and in a 29-O-ribose methylation event within the 18S rRNA.

Reactions in which C/D-box snoRNP is involved:
Pathways in which C/D-box snoRNP is involved:

Authors Title Journal
Kressler D, Linder P, de La Cruz J... Protein trans-acting factors involved in ribosome biogenesis in Saccharomyces cerevisiae. Mol Cell Biol 1999-12-01

Add your own comment!

There are no comments yet.

Last modification of this entry: April 16, 2013.

Welcome stranger! Click here to login or here to register.