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Bujnicki Lab Homepage

NGD in Saccharomyces cerevisiae

No-go decay

Proteins:
5'-3' exoribonuclease
Elongation factor 1 alpha-like protein
Polyadenylate-binding protein, cytoplasmic and nuclear
Protein DOM34
Enzymatic
complexes:
Exosome
Ribosome


Targets of NGD are poorly understood, but they appear to consist largely of mRNA transcripts on which ribosomes have stalled during translation. This stall can be caused by a variety of factors including strong secondary structures, which may physically block the translational machinery from moving down the transcript. Dom34/Hbs1 likely binds near the A site of stalled ribosomes and may facilitate recycling of complexes. In some cases, the transcript is also cleaved in an endonucleolytic fashion near the stall site; however the identity of the responsible endonuclease remains contentious. The fragmented mRNA molecules are then fully degraded by the exosome in a 3’ to 5’ fashion and by Xrn1 in a 5’ to 3’ fashion.




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Last modification of this entry: 2012-04-18 13:44:54
Edited by a user: kaja
Edited content: Changed rna_type.

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