biogenesis of stress-induced tRNA-derived RNAs
Stress-induced tRNA-derived RNAs (tiRNAs), found in diverse eukaryotes, generated by endonucleolytic cleavage in the anticodon loop of mature tRNAs, catalyzed by Rny1 in yeast [PMID: 19332891]. Under normal conditions Rny1 is sequestered in the vacuole away from its substrates [PMID: 19332891].
Rny1 is an RNase T2 family member, and is predicted to leave 5' hydroxyl and 2' -3' cyclic phosphate groups. This is unusual for intracellular cleavage and significant because the 5' hydroxyl group confers resistance to degradation by the 5' exonucleases Xrn1 and Rat1/Xrn2. Moreover, the tRNAsplicing ligase requires 5' hydroxyl and 2' -3' cyclic phosphate groups, suggesting that tRNA anticodon cleavage might be reversible under some circumstances.
19332891, "The RNase Rny1p cleaves tRNAs and promotes cell death during oxidative stress in Saccharomyces cerevisiae.", Thompson DM, 
21741109, "RNA in pieces.", Tuck AC, 
22492706, "Extensive terminal and asymmetric processing of small RNAs from rRNAs, snoRNAs, snRNAs, and tRNAs.", Li Z, 
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Last modification of this entry: 2012-08-18 13:37:48
Edited by a user: kaja
Edited content: Changed name, extended_name, rna_type and publications.