Known also as: Splicing endonuclease protein 2, tRNA-intron endonuclease SEN2
Known abbreviations: SEN2, YLR105C, L8004.12
Constitutes one of the two catalytic subunit of the tRNA-splicing endonuclease complex, a complex responsible for identification and cleavage of the splice sites in pre-tRNA. It cleaves pre-tRNA at the 5'- and 3'-splice sites to release the intron. The products are an intron and two tRNA half-molecules bearing 2',3'-cyclic phosphate and 5'-OH termini. There are no conserved sequences at the splice sites, but the intron is invariably located at the same site in the gene, placing the splice sites an invariant distance from the constant structural features of the tRNA body. This subunit may anchor the endonuclease complex to the nuclear membrane. Probably carries the active site for 5'-splice site cleavage.
Endonucleolytic cleavage of pre-tRNA, producing 5'-hydroxy and 2',3'-cyclic phosphate termini, and specifically removing the intron.
Heterotetramer composed of SEN2, SEN15, SEN34 and SEN54. Interacts directly with SEN54.
Nucleus. Endomembrane system; Peripheral membrane protein. Mitochondrion outer membrane; Peripheral membrane protein; Cytoplasmic side. Note: The tRNA splicing endonuclease complex is predominantly associated with the outer membrane of mitochondria, suggesting that tRNA splicing mainly takes place on the mitochondrial surface.
This protein can be a part of a given complexes:
Activities in which tRNA-splicing endonuclease subunit SEN2 is involved:
Pathways in which tRNA-splicing endonuclease subunit SEN2 is involved:
Links to other databases:
TRNA-splicing endonuclease subunit SEN2 (Saccharomyces cerevisiae) is product of expression of
TRNA-splicing endonuclease subunit SEN2 (Saccharomyces cerevisiae) belongs to following protein families:
||Publication date (Issue)
|The nucleotide sequence of Saccharomyces cerevisiae chromosome XII.
||Johnston M, Hillier L, Riles L, Albermann K, Andre B, Ansorge W, Benes V, Bruckner M, Delius H, Dubois E, Dusterhoft A, Entian KD, Floeth M, Goffeau A, Hebling U, Heumann K, Heuss-Neitzel D, Hilbert H, Hilger F, Kleine K, Kotter P, Louis EJ, Messenguy F, Mewes HW, Hoheisel JD, et al.
|The yeast tRNA splicing endonuclease: a tetrameric enzyme with two active site subunits homologous to the archaeal tRNA endonucleases.
||Trotta CR, Miao F, Arn EA, Stevens SW, Ho CK, Rauhut R, Abelson JN
|Global analysis of protein expression in yeast.
||Ghaemmaghami S, Huh WK, Bower K, Howson RW, Belle A, Dephoure N, O'Shea EK, Weissman JS
|Possibility of cytoplasmic pre-tRNA splicing: the yeast tRNA splicing endonuclease mainly localizes on the mitochondria.
||Yoshihisa T, Yunoki-Esaki K, Ohshima C, Tanaka N, Endo T
Mol Biol Cell
|Yeast tRNA-splicing endonuclease is a heterotrimeric enzyme.
||Rauhut R, Green PR, Abelson J
J Biol Chem
|Accumulation of pre-tRNA splicing '2/3' intermediates in a Saccharomyces cerevisiae mutant.
||Ho CK, Rauhut R, Vijayraghavan U, Abelson J
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Last modification of this entry: Sept. 25, 2012.