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tRNA-splicing endonuclease subunit SEN15

 
Known also as: Splicing endonuclease of 15 kDa, tRNA-intron endonuclease SEN15

Known abbreviations: SEN15, YMR059W, YM9796.12

 

FUNCTION:
 
Non-catalytic subunit of the tRNA-splicing endonuclease complex, a complex responsible for identification and cleavage of the splice sites in pre-tRNA. It cleaves pre-tRNA at the 5' and 3' splice sites to release the intron. The products are an intron and two tRNA half-molecules bearing 2',3' cyclic phosphate and 5'-OH termini. There are no conserved sequences at the splice sites, but the intron is invariably located at the same site in the gene, placing the splice sites an invariant distance from the constant structural features of the tRNA body.
 
SUBUNIT STRUCTURE:
 
tRNA splicing endonuclease is a heterotetramer composed of SEN2, SEN15, SEN34 and SEN54. Interacts directly with SEN34. 
 
CELLULAR LOCALIZATION:
 
Nucleus. Endomembrane system; Peripheral membrane protein. Mitochondrion outer membrane; Peripheral membrane protein; Cytoplasmic side. Note: The tRNA splicing endonuclease complex is predominantly associated with the outer membrane of mitochondria, suggesting that tRNA splicing mainly takes place on the mitochondrial surface. 



This protein can be a part of a given complexes: Activities in which tRNA-splicing endonuclease subunit SEN15 is involved: Pathways in which tRNA-splicing endonuclease subunit SEN15 is involved:

NCBI GI number(s): 99030929
296147177
Species: Saccharomyces cerevisiae

Links to other databases:

Database ID Link
Uniprot Q04675 Q04675
BRENDA - -
KEGG sce:YMR059W sce:YMR059W
PFAM: PF09631
PF09631
InterPro: IPR018593
IPR018593
CATH: - -
SCOP: - -


Protein sequence:
MATTDIISLVKNNLLYFQMWTEVEILQDDLSWKGNSLRLLRGRPPHKLSN
DVDTEHENSLSSPRPLEFILPINMSQYKENFLTLECLSQTFTHLCSPSTE
RILLAIINDDGTIVYYFVYKGVRKPKRN

TRNA-splicing endonuclease subunit SEN15 (Saccharomyces cerevisiae) is product of expression of SEN15 gene.

References:

Title Authors Journal Publication date (Issue) PubMed ID
The nucleotide sequence of Saccharomyces cerevisiae chromosome XIII. Bowman S, Churcher C, Badcock K, Brown D, Chillingworth T, Connor R, Dedman K, Devlin K, Gentles S, Hamlin N, Hunt S, Jagels K, Lye G, Moule S, Odell C, Pearson D, Rajandream M, Rice P, Skelton J, Walsh S, Whitehead S, Barrell B Nature 1997-05-01 (387) 9169872
The yeast tRNA splicing endonuclease: a tetrameric enzyme with two active site subunits homologous to the archaeal tRNA endonucleases. Trotta CR, Miao F, Arn EA, Stevens SW, Ho CK, Rauhut R, Abelson JN Cell 1997-06-13 (89) 9200603
Possibility of cytoplasmic pre-tRNA splicing: the yeast tRNA splicing endonuclease mainly localizes on the mitochondria. Yoshihisa T, Yunoki-Esaki K, Ohshima C, Tanaka N, Endo T Mol Biol Cell 2003-08-01 (14) 12925762



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Last modification of this entry: Sept. 25, 2012.

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