Known also as: Splicing endonuclease of 34 kDa, tRNA-intron endonuclease SEN34
Known abbreviations: SEN34, YAR008W, FUN4
Constitutes one of the two catalytic subunit of the tRNA-splicing endonuclease complex, a complex responsible for identification and cleavage of the splice sites in pre-tRNA. It cleaves pre-tRNA at the 5'- and 3'-splice sites to release the intron. The products are an intron and two tRNA half-molecules bearing 2',3'-cyclic phosphate and 5'-OH termini. There are no conserved sequences at the splice sites, but the intron is invariably located at the same site in the gene, placing the splice sites an invariant distance from the constant structural features of the tRNA body. It probably carries the active site for 3'-splice site cleavage.
Endonucleolytic cleavage of pre-tRNA, producing 5'-hydroxy and 2',3'-cyclic phosphate termini, and specifically removing the intron.
Heterotetramer composed of SEN2, SEN15, SEN34 and SEN54. Interacts directly with SEN15.
Nucleus. Endomembrane system; Peripheral membrane protein. Mitochondrion outer membrane; Peripheral membrane protein; Cytoplasmic side. Note: The tRNA splicing endonuclease complex is predominantly associated with the outer membrane of mitochondria, suggesting that tRNA splicing mainly takes place on the mitochondrial surface.
This protein can be a part of a given complexes:
Activities in which tRNA-splicing endonuclease subunit SEN34 is involved:
Pathways in which tRNA-splicing endonuclease subunit SEN34 is involved:
Links to other databases:
TRNA-splicing endonuclease subunit SEN34 (Saccharomyces cerevisiae) is product of expression of
TRNA-splicing endonuclease subunit SEN34 (Saccharomyces cerevisiae) belongs to following protein families:
||Publication date (Issue)
|The nucleotide sequence of chromosome I from Saccharomyces cerevisiae.
||Bussey H, Kaback DB, Zhong W, Vo DT, Clark MW, Fortin N, Hall J, Ouellette BF, Keng T, Barton AB, et al.
Proc Natl Acad Sci U S A
|The yeast tRNA splicing endonuclease: a tetrameric enzyme with two active site subunits homologous to the archaeal tRNA endonucleases.
||Trotta CR, Miao F, Arn EA, Stevens SW, Ho CK, Rauhut R, Abelson JN
|Sequencing of chromosome I of Saccharomyces cerevisiae: analysis of the 42 kbp SPO7-CENI-CDC15 region.
||Clark MW, Keng T, Storms RK, Zhong W, Fortin N, Zeng B, Delaney S, Ouellette BF, Barton AB, Kaback DB, et al.
|Possibility of cytoplasmic pre-tRNA splicing: the yeast tRNA splicing endonuclease mainly localizes on the mitochondria.
||Yoshihisa T, Yunoki-Esaki K, Ohshima C, Tanaka N, Endo T
Mol Biol Cell
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Last modification of this entry: Sept. 25, 2012.