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mRNA-capping enzyme subunit beta

 
Known also as: Polynucleotide 5'-triphosphatase, mRNA 5'-triphosphatase

Known abbreviations: TPase, CET1, YPL228W, P1433

 

FUNCTION:
 
First step of mRNA capping. Converts the 5'-triphosphate end of a nascent mRNA chain into a diphosphate end. 
 
CATALYTIC ACTIVITY:
 
A 5'-phosphopolynucleotide + H2O = a polynucleotide + phosphate.
 
COFACTOR:
 
Divalent ions.
 
SUBUNIT STRUCTURE:
 
The mRNA-capping enzyme is composed of two separate chains alpha and beta, respectively a mRNA guanylyltransferase and an RNA 5'-triphosphatase. 
 
CELLULAR LOCALIZATION:
 
Nucleus.



Activities in which mRNA-capping enzyme subunit beta is involved: Pathways in which mRNA-capping enzyme subunit beta is involved:

NCBI GI number(s): 6325028
296148431
Species: Saccharomyces cerevisiae

Links to other databases:

Database ID Link
Uniprot O13297 O13297
BRENDA - -
KEGG sce:YPL228W sce:YPL228W
PFAM: PF02940
PF02940
InterPro: IPR023577
IPR004206
IPR023577
IPR004206
CATH: - -
SCOP: - -
Solved crystal structures: 3KYH
1D8I
1D8H
[PDB] [details]
[PDB] [details]
[PDB] [details]


Protein sequence:
MSYTDNPPQTKRALSLDDLVNHDENEKVKLQKLSEAANGSRPFAENLESD
INQTETGQAAPIDNYKESTGHGSHSQKPKSRKSSNDDEETDTDDEMGASG
EINFDSEMDFDYDKQHRNLLSNGSPPMNDGSDANAKLEKPSDDSIHQNSK
SDEEQRIPKQGNEGNIASNYITQVPLQKQKQTEKKIAGNAVGSVVKKEEE
ANAAVDNIFEEKATLQSKKNNIKRDLEVLNEISASSKPSKYRNVPIWAQK
WKPTIKALQSINVKDLKIDPSFLNIIPDDDLTKSVQDWVYATIYSIAPEL
RSFIELEMKFGVIIDAKGPDRVNPPVSSQCVFTELDAHLTPNIDASLFKE
LSKYIRGISEVTENTGKFSIIESQTRDSVYRVGLSTQRPRFLRMSTDIKT
GRVGQFIEKRHVAQLLLYSPKDSYDVKISLNLELPVPDNDPPEKYKSQSP
ISERTKDRVSYIHNDSCTRIDITKVENHNQNSKSRQSETTHEVELEINTP
ALLNAFDNITNDSKEYASLIRTFLNNGTIIRRKLSSLSYEIFEGSKKVM

MRNA-capping enzyme subunit beta (Saccharomyces cerevisiae) is product of expression of CET1 gene.

References:

Title Authors Journal Publication date (Issue) PubMed ID
The nucleotide sequence of Saccharomyces cerevisiae chromosome XVI. Bussey H, Storms RK, Ahmed A, Albermann K, Allen E, Ansorge W, Araujo R, Aparicio A, Barrell B, Badcock K, Benes V, Botstein D, Bowman S, Bruckner M, Carpenter J, Cherry JM, Chung E, Churcher C, Coster F, Davis K, Davis RW, Dietrich FS, Delius H, DiPaolo T, Hani J, et al. Nature 1997-05-01 (387) 9169875
Global analysis of protein expression in yeast. Ghaemmaghami S, Huh WK, Bower K, Howson RW, Belle A, Dephoure N, O'Shea EK, Weissman JS Nature 2003-10-16 (425) 14562106
Large-scale phosphorylation analysis of alpha-factor-arrested Saccharomyces cerevisiae. Li X, Gerber SA, Rudner AD, Beausoleil SA, Haas W, Villen J, Elias JE, Gygi SP J Proteome Res 2007-03-01 (6) 17330950
A multidimensional chromatography technology for in-depth phosphoproteome analysis. Albuquerque CP, Smolka MB, Payne SH, Bafna V, Eng J, Zhou H Mol Cell Proteomics 2008-07-01 (7) 18407956
Proteome-wide identification of in vivo targets of DNA damage checkpoint kinases. Smolka MB, Albuquerque CP, Chen SH, Zhou H Proc Natl Acad Sci U S A 2007-06-19 (104) 17563356
Analysis of phosphorylation sites on proteins from Saccharomyces cerevisiae by electron transfer dissociation (ETD) mass spectrometry. Chi A, Huttenhower C, Geer LY, Coon JJ, Syka JE, Bai DL, Shabanowitz J, Burke DJ, Troyanskaya OG, Hunt DF Proc Natl Acad Sci U S A 2007-01-13 (104) 17287358
Messenger RNA guanylyltransferase from Saccharomyces cerevisiae. I. Purification and subunit structure. Itoh N, Mizumoto K, Kaziro Y J Biol Chem 1984-11-25 (259) 6389537
Messenger RNA guanlyltransferase from Saccharomyces cerevisiae. II. Catalytic properties. Itoh N, Mizumoto K, Kaziro Y J Biol Chem 1984-11-25 (259) 6094533
Messenger RNA guanylyltransferase from Saccharomyces cerevisiae. Large scale purification, subunit functions, and subcellular localization. Itoh N, Yamada H, Kaziro Y, Mizumoto K J Biol Chem 1987-01-15 (262) 3029058
Isolation and characterization of the yeast mRNA capping enzyme beta subunit gene encoding RNA 5'-triphosphatase, which is essential for cell viability. Tsukamoto T, Shibagaki Y, Imajoh-Ohmi S, Murakoshi T, Suzuki M, Nakamura A, Gotoh H, Mizumoto K Biochem Biophys Res Commun 1997-10-09 (239) 9345280
Homodimeric quaternary structure is required for the in vivo function and thermal stability of Saccharomyces cerevisiae and Schizosaccharomyces pombe RNA triphosphatases. Hausmann S, Pei Y, Shuman S J Biol Chem 2003-08-15 (278) 12788946
Structure and mechanism of yeast RNA triphosphatase: an essential component of the mRNA capping apparatus. Lima CD, Wang LK, Shuman S Cell 1999-11-24 (99) 10589681



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Last modification of this entry: Sept. 25, 2012.

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