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mRNA-capping enzyme subunit beta |
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Known abbreviations: TPase, CET1, YPL228W, P1433
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FUNCTION:
First step of mRNA capping. Converts the 5'-triphosphate end of a nascent mRNA chain into a diphosphate end.
CATALYTIC ACTIVITY:
A 5'-phosphopolynucleotide + H2O = a polynucleotide + phosphate.
COFACTOR:
Divalent ions.
SUBUNIT STRUCTURE:
The mRNA-capping enzyme is composed of two separate chains alpha and beta, respectively a mRNA guanylyltransferase and an RNA 5'-triphosphatase.
CELLULAR LOCALIZATION:
Nucleus.
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Activities in which mRNA-capping enzyme subunit beta is involved:
- Hydrolysis of the 5' end of the nascent transcript by the capping enzyme in Saccharomyces cerevisiae [reaction id: 200]
- Recognition of the 5' end of the nascent transcript by the capping enzyme in Saccharomyces cerevisiae [reaction id: 239]
| NCBI GI number(s): |
6325028 296148431 |
| Species: | Saccharomyces cerevisiae |
Links to other databases:
| Database | ID | Link |
|---|---|---|
| Uniprot | O13297 | O13297 |
| BRENDA | - | - |
| KEGG | sce:YPL228W | sce:YPL228W |
| PFAM: |
PF02940 |
PF02940 |
| InterPro: |
IPR023577 IPR004206 |
IPR023577 IPR004206 |
| CATH: | - | - |
| SCOP: | - | - |
| Solved crystal structures: |
3KYH 1D8I 1D8H |
[PDB]
[details] [PDB] [details] [PDB] [details] |
Protein sequence:
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MSYTDNPPQTKRALSLDDLVNHDENEKVKLQKLSEAANGSRPFAENLESD INQTETGQAAPIDNYKESTGHGSHSQKPKSRKSSNDDEETDTDDEMGASG EINFDSEMDFDYDKQHRNLLSNGSPPMNDGSDANAKLEKPSDDSIHQNSK SDEEQRIPKQGNEGNIASNYITQVPLQKQKQTEKKIAGNAVGSVVKKEEE ANAAVDNIFEEKATLQSKKNNIKRDLEVLNEISASSKPSKYRNVPIWAQK WKPTIKALQSINVKDLKIDPSFLNIIPDDDLTKSVQDWVYATIYSIAPEL RSFIELEMKFGVIIDAKGPDRVNPPVSSQCVFTELDAHLTPNIDASLFKE LSKYIRGISEVTENTGKFSIIESQTRDSVYRVGLSTQRPRFLRMSTDIKT GRVGQFIEKRHVAQLLLYSPKDSYDVKISLNLELPVPDNDPPEKYKSQSP ISERTKDRVSYIHNDSCTRIDITKVENHNQNSKSRQSETTHEVELEINTP ALLNAFDNITNDSKEYASLIRTFLNNGTIIRRKLSSLSYEIFEGSKKVM |
MRNA-capping enzyme subunit beta (Saccharomyces cerevisiae) is product of expression of CET1 gene.
References:
| Title | Authors | Journal | Publication date (Issue) | PubMed ID |
|---|---|---|---|---|
| The nucleotide sequence of Saccharomyces cerevisiae chromosome XVI. | Bussey H, Storms RK, Ahmed A, Albermann K, Allen E, Ansorge W, Araujo R, Aparicio A, Barrell B, Badcock K, Benes V, Botstein D, Bowman S, Bruckner M, Carpenter J, Cherry JM, Chung E, Churcher C, Coster F, Davis K, Davis RW, Dietrich FS, Delius H, DiPaolo T, Hani J, et al. | Nature | 1997-05-01 (387) | 9169875 |
| Global analysis of protein expression in yeast. | Ghaemmaghami S, Huh WK, Bower K, Howson RW, Belle A, Dephoure N, O'Shea EK, Weissman JS | Nature | 2003-10-16 (425) | 14562106 |
| Large-scale phosphorylation analysis of alpha-factor-arrested Saccharomyces cerevisiae. | Li X, Gerber SA, Rudner AD, Beausoleil SA, Haas W, Villen J, Elias JE, Gygi SP | J Proteome Res | 2007-03-01 (6) | 17330950 |
| A multidimensional chromatography technology for in-depth phosphoproteome analysis. | Albuquerque CP, Smolka MB, Payne SH, Bafna V, Eng J, Zhou H | Mol Cell Proteomics | 2008-07-01 (7) | 18407956 |
| Proteome-wide identification of in vivo targets of DNA damage checkpoint kinases. | Smolka MB, Albuquerque CP, Chen SH, Zhou H | Proc Natl Acad Sci U S A | 2007-06-19 (104) | 17563356 |
| Analysis of phosphorylation sites on proteins from Saccharomyces cerevisiae by electron transfer dissociation (ETD) mass spectrometry. | Chi A, Huttenhower C, Geer LY, Coon JJ, Syka JE, Bai DL, Shabanowitz J, Burke DJ, Troyanskaya OG, Hunt DF | Proc Natl Acad Sci U S A | 2007-01-13 (104) | 17287358 |
| Messenger RNA guanylyltransferase from Saccharomyces cerevisiae. I. Purification and subunit structure. | Itoh N, Mizumoto K, Kaziro Y | J Biol Chem | 1984-11-25 (259) | 6389537 |
| Messenger RNA guanlyltransferase from Saccharomyces cerevisiae. II. Catalytic properties. | Itoh N, Mizumoto K, Kaziro Y | J Biol Chem | 1984-11-25 (259) | 6094533 |
| Messenger RNA guanylyltransferase from Saccharomyces cerevisiae. Large scale purification, subunit functions, and subcellular localization. | Itoh N, Yamada H, Kaziro Y, Mizumoto K | J Biol Chem | 1987-01-15 (262) | 3029058 |
| Isolation and characterization of the yeast mRNA capping enzyme beta subunit gene encoding RNA 5'-triphosphatase, which is essential for cell viability. | Tsukamoto T, Shibagaki Y, Imajoh-Ohmi S, Murakoshi T, Suzuki M, Nakamura A, Gotoh H, Mizumoto K | Biochem Biophys Res Commun | 1997-10-09 (239) | 9345280 |
| Homodimeric quaternary structure is required for the in vivo function and thermal stability of Saccharomyces cerevisiae and Schizosaccharomyces pombe RNA triphosphatases. | Hausmann S, Pei Y, Shuman S | J Biol Chem | 2003-08-15 (278) | 12788946 |
| Structure and mechanism of yeast RNA triphosphatase: an essential component of the mRNA capping apparatus. | Lima CD, Wang LK, Shuman S | Cell | 1999-11-24 (99) | 10589681 |
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Last modification of this entry: Sept. 25, 2012.
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