Modomics - A Database of RNA Modifications

ID Card:

Full name: 28S rRNA (cytosine-C(5))-methyltransferase
UniProt: Q96P11
Alpha Fold Predicted Structure: AF-Q96P11-F1
Enzyme type: methyltransferase



Protein sequence:

MGLYAAAAGVLAGVESRQGSIKGLVYSSNFQNVKQLYALVCETQRYSAVLDAVIASAGLLRAEKKLRPHLAKVLVYELLLGKGFRGGGGRWKALLGRHQARLKAELARLKVHRGVSRNEDLLEVGSRPGPASQLPRFVRVNTLKTCSDDVVDYFKRQGFSYQGRASSLDDLRALKGKHFLLDPLMPELLVFPAQTDLHEHPLYRAGHLILQDRASCLPAMLLDPPPGSHVIDACAAPGNKTSHLAALLKNQGKIFAFDLDAKRLASMATLLARAGVSCCELAEEDFLAVSPSDPRYHEVHYILLDPSCSGSGMPSRQLEEPGAGTPSPVRLHALAGFQQRALCHALTFPSLQRLVYSTCSLCQEENEDVVRDALQQNPGAFRLAPALPAWPHRGLSTFPGAEHCLRASPETTLSSGFFVAVIERVEVPR

Comments:

S-adenosyl-L-methionine-dependent methyltransferase that specifically methylates the C5 position of cytosine 3782 (m5C3782) in 28S rRNA (Sharma et al. 2013 , Heissenberger et al. 2019 ) . m5C3782 promotes protein translation without affecting ribosome biogenesis and fidelity (Janin et al. 2019 , Heissenberger et al. 2019 )





Alpha Fold Predicted Structure:






Clear Selection and Reset Camera

Protein sequence:

M G L Y A A A A G V L A G V E S R Q G S I K G L V Y S S N F Q N V K Q L Y A L V C E T Q R Y S A V L D A V I A S A G L L R A E K K L R P H L A K V L V Y E L L L G K G F R G G G G R W K A L L G R H Q A R L K A E L A R L K V H R G V S R N E D L L E V G S R P G P A S Q L P R F V R V N T L K T C S D D V V D Y F K R Q G F S Y Q G R A S S L D D L R A L K G K H F L L D P L M P E L L V F P A Q T D L H E H P L Y R A G H L I L Q D R A S C L P A M L L D P P P G S H V I D A C A A P G N K T S H L A A L L K N Q G K I F A F D L D A K R L A S M A T L L A R A G V S C C E L A E E D F L A V S P S D P R Y H E V H Y I L L D P S C S G S G M P S R Q L E E P G A G T P S P V R L H A L A G F Q Q R A L C H A L T F P S L Q R L V Y S T C S L C Q E E N E D V V R D A L Q Q N P G A F R L A P A L P A W P H R G L S T F P G A E H C L R A S P E T T L S S G F F V A V I E R V E V P R

Secondary Structure Alphabet

  • G: 3-turn helix (310helix)
  • H: α-helix
  • I: 𝝅-helix (5 - turn helix)
  • T: Hydrogen Bonded Turn
  • B: β-sheet
  • S: Bend
  • C: Coil (residues not present in any of the above conformations)
  • N: Not assigned

Download PDB Structures & DSSP Secondary Structures:

Alpha Fold Pdb Files   AF-Q96P11-F1.pdb  
Alpha Fold Pdbx/mmCIF Files   AF-Q96P11-F1.cif  
DSSP Secondary Structures   Q96P11.dssp  





Publications:

Title Authors Journal Details PubMed Id DOI
Yeast Nop2 and Rcm1 methylate C2870 and C2278 of the 25S rRNA, respectively Sunny Sharma 1, Jun Yang, Peter Watzinger, Peter Kötter, Karl-Dieter Entian [details] 23913415 10.1093/nar/gkt679
Loss of the ribosomal RNA methyltransferase NSUN5 impairs global protein synthesis and normal growth Clemens Heissenberger 1, Lisa Liendl 1, Fabian Nagelreiter 1, Yulia Gonskikh 2, Guohuan Yang 3, Elena M Stelzer 1, Teresa L Krammer 1, Lucia Micutkova 4, Stefan Vogt 1, David P Kreil 1, Gerhard Sekot 1, Emilio Siena 1, Ina Poser 5, Eva Harreither 1, Angela Linder 1, Viktoria Ehret 6, Thomas H Helbich 6, Regina Grillari-Voglauer 1, Pidder Jansen-Dürr 4, Martin Koš 3, Norbert Polacek 2, Johannes Grillari 1 7 8, Markus Schosserer [details] 31722427 10.1093/nar/gkz1043
Epigenetic loss of RNA-methyltransferase NSUN5 in glioma targets ribosomes to drive a stress adaptive translational program Maxime Janin 1, Vanessa Ortiz-Barahona 1, Manuel Castro de Moura 1, Anna Martínez-Cardús 1, Pere Llinàs-Arias 1, Marta Soler 1, Daphna Nachmani 2, Joffrey Pelletier 3, Ulrike Schumann 4, Maria E Calleja-Cervantes 1, Sebastian Moran 1, Sonia Guil 1, Alberto Bueno-Costa 1, David Piñeyro 1, Montserrat Perez-Salvia 1, Margalida Rosselló-Tortella 1, Laia Piqué 1, Joan J Bech-Serra 5, Carolina De La Torre 5, August Vidal 6 7, María Martínez-Iniesta 8, Juan F Martín-Tejera 8, Alberto Villanueva 8, Alexandra Arias 9, Isabel Cuartas 9, Ana M Aransay 10, Andres Morales La Madrid 11 12, Angel M Carcaboso 13, Vicente Santa-Maria 11 12, Jaume Mora 11, Agustin F Fernandez 14, Mario F Fraga 15, Iban Aldecoa 16, Leire Pedrosa 17, Francesc Graus 18, Noemi Vidal 6, Fina Martínez-Soler 19, Avelina Tortosa 19, Cristina Carrato 20, Carme Balañá 21, Matthew W Boudreau 22, Paul J Hergenrother 22, Peter Kötter 23, Karl-Dieter Entian 23, Jürgen Hench 24, Stephan Frank 24, Sheila Mansouri 25, Gelareh Zadeh 25, Pablo D Dans 26, Modesto Orozco 26 27, George Thomas 3 28 29, Sandra Blanco 30 31, Joan Seoane 7 9 32, Thomas Preiss 4 33, Pier Paolo Pandolfi 2, Manel Esteller [details] 31428936 10.1007/s00401-019-02062-4

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