Modomics - A Database of RNA Modifications

ID Card:

Full name: 26S rRNA (cytosine-C(5))-methyltransferase nsun-5
UniProt: Q9NAA7
Alpha Fold Predicted Structure: AF-Q9NAA7-F1
Enzyme type: methyltransferase



Protein sequence:

MATDALYNEVAEIIRCVLAKEKSVRNAVYGSSYKNKKALLRLSCESLKFRPVFDEILQDKELKSMKRDANIGGSVELLYVLMYETLVGSGLTRCSQELKSVISRRIQRIKEVEHAMQDEGRGIKAMKEADDGMKKIQIPRYARINTLKWTADEAMKTLETEKWKILGTLKPENFAEMVTKMKDDEVYVDPHVENLIIFAPNIQNFYEYWMVEQRYLILQDKASCLPAFLLNPRPGSQVFDTCAAPGMKTSHAAAIMENQGKVWAMDRAADRVATMKQLLDASKVAIASSFCGDFLKTDVTDKKFSKVKFAIVDPPCSGSGIVKRMDEITGGNAEKERLEKLKNLQAMILKHALKLPGLKRAVYSTCSVHEEENEQVVDEVLLDTYVRQNYVLKKNVLPEWTYRGLKTYEVGEHCLRANPKVTLTNGFFVAVFERVKSSE

Comments:

S-adenosyl-L-methionine-dependent methyltransferase which methylates the carbon-5 position of cytosine 2381 to 5-methylcytosine (m5C2381) in 26S rRNA (Schosserer et al. 2015, Heissenberger et al. 2020 , Navarro et al. 2021 ). Plays a role in the production of mature 5S, 5.8S, 18S and 26S rRNAs and promotes the processing of the internally transcribed spacer 2 (ITS2), which separates the 5.8S and 26S rRNAs on large pre-rRNA precursors (Heissenberger et al. 2020 ,).





Alpha Fold Predicted Structure:






Clear Selection and Reset Camera

Protein sequence:

M A T D A L Y N E V A E I I R C V L A K E K S V R N A V Y G S S Y K N K K A L L R L S C E S L K F R P V F D E I L Q D K E L K S M K R D A N I G G S V E L L Y V L M Y E T L V G S G L T R C S Q E L K S V I S R R I Q R I K E V E H A M Q D E G R G I K A M K E A D D G M K K I Q I P R Y A R I N T L K W T A D E A M K T L E T E K W K I L G T L K P E N F A E M V T K M K D D E V Y V D P H V E N L I I F A P N I Q N F Y E Y W M V E Q R Y L I L Q D K A S C L P A F L L N P R P G S Q V F D T C A A P G M K T S H A A A I M E N Q G K V W A M D R A A D R V A T M K Q L L D A S K V A I A S S F C G D F L K T D V T D K K F S K V K F A I V D P P C S G S G I V K R M D E I T G G N A E K E R L E K L K N L Q A M I L K H A L K L P G L K R A V Y S T C S V H E E E N E Q V V D E V L L D T Y V R Q N Y V L K K N V L P E W T Y R G L K T Y E V G E H C L R A N P K V T L T N G F F V A V F E R V K S S E

Secondary Structure Alphabet

  • G: 3-turn helix (310helix)
  • H: α-helix
  • I: 𝝅-helix (5 - turn helix)
  • T: Hydrogen Bonded Turn
  • B: β-sheet
  • S: Bend
  • C: Coil (residues not present in any of the above conformations)
  • N: Not assigned

Download PDB Structures & DSSP Secondary Structures:

Alpha Fold Pdb Files   AF-Q9NAA7-F1.pdb  
Alpha Fold Pdbx/mmCIF Files   AF-Q9NAA7-F1.cif  
DSSP Secondary Structures   Q9NAA7.dssp  





Publications:

Title Authors Journal Details PubMed Id DOI
Methylation of ribosomal RNA by NSUN5 is a conserved mechanism modulating organismal lifespan. Schosserer M, Minois N, Angerer TB, Amring M, Dellago H, Harreither E, Calle-Perez A, Pircher A, Gerstl MP, Pfeifenberger S, Brandl C, Sonntagbauer M, Kriegner A, Linder A, Weinhausel A, Mohr T, Steiger M, Mattanovich D, Rinnerthaler M, Karl T, Sharma S, Entian KD, Kos M, Breitenbach M, Wilson IB, Polacek N, Grillari-Voglauer R, Breitenbach-Koller L, Grillari J... Nat Commun [details] 25635753 -
The ribosomal RNA m5C methyltransferase NSUN-1 modulates healthspan and oogenesis in Caenorhabditis elegans 33289480 [details] 33289480 10.7554/eLife.56205
Translational adaptation to heat stress is mediated by RNA 5-methylcytosine in Caenorhabditis elegans [details] 33283887 10.15252/embj.2020105496

Links:

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