Modomics - A Database of RNA Modifications

ID Card:

Full name: U6 small nuclear RNA (adenine-(43)-N(6))-methyltransferase
UniProt: F4IGH3
Alpha Fold Predicted Structure: AF-F4IGH3-F1
Enzyme type: methyltransferase



Protein sequence:

MRSGKKRARSEPGSTKQPRSKYSDNPPDFASLASLYPSFKPFVFFSGSRARIDWTDYNATRELTRVLLLHDHGVNWWIPDGQLCPTVPNRSNYIHWINDLLSSGIIQSLGGDGSKVKGFDIGTGANCIYPLLGASLFGWSFVGSDFTVVALEWAEKNVQSNPHFSDLIEIRDSKVPPQCSSVPEVENTEREKTIQEEAEISATVKSDYHDNKSFIEPAVLLGVVKENETFDFCMSNPPFFETFEEAGLNPKTSCGGTPEEMVCNGGEQAFVSRIIKDSAVLRQRFRWYTSMLGKKANLKLLISKLWEVGVTIVKTTEFVQGQTSRWGLAWSFMPIARKIIAPPVVKKSVLSFMLECIKRQYSAVDVLQSVEEFFKSCGASCKLNSSTFSVDIVASNDQCNTISKNDIADVDSVRSHGYEKQSLDGSSLQVPEYNLSFRILVFQQMPGTLLIKGSLQQKDSPLSGLFSRVFGSLEESLKSKFCRLTLRGKIHRAKLDYPKVVKERERERVLHFT

Comments:

FIONA1 installs m6A modification in U6 snRNA and a small subset of poly(A)+ RNA in Arabidopsis. FIONA1-mediated m6A post-transcriptional regulation is an autonomous regulator for flowering and phytochrome signaling-dependent photomorphogenesis.





Alpha Fold Predicted Structure:






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Protein sequence:

M R S G K K R A R S E P G S T K Q P R S K Y S D N P P D F A S L A S L Y P S F K P F V F F S G S R A R I D W T D Y N A T R E L T R V L L L H D H G V N W W I P D G Q L C P T V P N R S N Y I H W I N D L L S S G I I Q S L G G D G S K V K G F D I G T G A N C I Y P L L G A S L F G W S F V G S D F T V V A L E W A E K N V Q S N P H F S D L I E I R D S K V P P Q C S S V P E V E N T E R E K T I Q E E A E I S A T V K S D Y H D N K S F I E P A V L L G V V K E N E T F D F C M S N P P F F E T F E E A G L N P K T S C G G T P E E M V C N G G E Q A F V S R I I K D S A V L R Q R F R W Y T S M L G K K A N L K L L I S K L W E V G V T I V K T T E F V Q G Q T S R W G L A W S F M P I A R K I I A P P V V K K S V L S F M L E C I K R Q Y S A V D V L Q S V E E F F K S C G A S C K L N S S T F S V D I V A S N D Q C N T I S K N D I A D V D S V R S H G Y E K Q S L D G S S L Q V P E Y N L S F R I L V F Q Q M P G T L L I K G S L Q Q K D S P L S G L F S R V F G S L E E S L K S K F C R L T L R G K I H R A K L D Y P K V V K E R E R E R V L H F T

Secondary Structure Alphabet

  • G: 3-turn helix (310helix)
  • H: α-helix
  • I: 𝝅-helix (5 - turn helix)
  • T: Hydrogen Bonded Turn
  • B: β-sheet
  • S: Bend
  • C: Coil (residues not present in any of the above conformations)
  • N: Not assigned

Download PDB Structures & DSSP Secondary Structures:

Alpha Fold Pdb Files   AF-F4IGH3-F1.pdb  
Alpha Fold Pdbx/mmCIF Files   AF-F4IGH3-F1.cif  
DSSP Secondary Structures   F4IGH3.dssp  





Publications:

Title Authors Journal Details PubMed Id DOI
FIONA1 is an RNA N6-methyladenosine methyltransferase affecting Arabidopsis photomorphogenesis and flowering Chunling Wang # 1, Junbo Yang # 1, Peizhe Song 1, Wei Zhang 1, Qiang Lu 1, Qiong Yu 1, Guifang Jia [details] 35101091 10.1186/s13059-022-02612-2
Arabidopsis N6-methyladenosine methyltransferase FIONA1 regulates floral transition by affecting the splicing of FLC and the stability of floral activators SPL3 and SEP3 Jing Cai 1, Jianzhong Hu 1, Umme Amara 1, Su Jung Park 1, Yuxia Li 2, Daesong Jeong 3, Ilha Lee 3, Tao Xu 2, Hunseung Kang [details] 36416766 10.1093/jxb/erac461
FIONA1-Mediated m6 A Modification Regulates the Floral Transition in Arabidopsis Tao Xu 1 2, Xiaowei Wu 1 2, Chui Eng Wong 1 2, Sheng Fan 2, Yu Zhang 1 2, Songyao Zhang 1, Zhe Liang 1 3, Hao Yu 1 2, Lisha Shen 2 [details] 34989479 10.1002/advs.202103628
m6A modification of U6 snRNA modulates usage of two major classes of pre-mRNA 5' splice site Matthew T Parker 1, Beth K Soanes 2, Jelena Kusakina 2, Antoine Larrieu 2, Katarzyna Knop 1, Nisha Joy 1, Friedrich Breidenbach 1 3, Anna V Sherwood 1, Geoffrey J Barton 1, Sebastian M Fica 4, Brendan H Davies 2, Gordon G Simpson 1 5 [details] 36409063 10.7554/eLife.78808