DNAmoreDB - A Database of Deoxyribozymes

DNAzyme description

 Reaction  Reacting groups  Substrates Product  Metal ion Linkage  Seq description
RNA ligation Group 1 - 2',3 '- diol

Group 2 - 5'-triphosphate

L: GGAACUGCGAUCUAGUGA
R: GACUGACUCGUGAUCGGA
native RNA Mn2+
Mg2+
3',5' N 22
 Buffer conditions
50 mM HEPES pH 7.5, 150 mM NaCl, 5 mM KCl, 40 mM MgCl2, 20 mM MnCl2, 1 mM atrazine, 10% methanol
 Catalytic region of the DNAzyme
GGACCATGGGGGAGCGGCCCGC
Notes
In vitro selections aim at identifying RNA-ligating deoxyribozymes that depend on a target herbicide for activity. The selections denoted 'Q' used alachlor, while the selection denoted 'R' used atrazine.

 Reported in...

Year of Publication First Author Laboratory Title PubMed ID DOI Reaction
2013 A K Behera A Baum Enhanced deoxyribozyme‐catalyzed RNA ligation in the presence of organic cosolvents None 10.1002/bip.22191 RNA ligation

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