DNAmoreDB - A Database of Deoxyribozymes

DNAzyme description

 Reaction  Reacting groups  Substrates Product  Metal ion Linkage  Seq description
RNA cleavage Group 1 - 2'-OH of rA

Group 2 - vicinal phosphate

S: *
specific cleavage at desired position Lu3+
RNA phosphodiester N 35
 Buffer conditions
50 mM MES pH 6.0, 25 mM NaCl, Lu3+
 Catalytic region of the DNAzyme
CTGCAGAATTCTAATACGAGTCACTATAGGAAGATGGCGAAACATCTTTACAAGCATCAGTAGATTGGAAATGCGGTTATAGTGACGGTAAGCTTGGCAC
Notes
This is the cis-cleaving version of the DNAzyme. Sequences have been annotated as reported. The cleavage site rA is in position 28 in the reported sequence. The thymine at position 48 is the starting of the N35 region.

 Reported in...

Year of Publication First Author Laboratory Title PubMed ID DOI Reaction
2014 P-J J Huang J Liu In vitro selection of a new lanthanide-dependent DNAzyme for ratiometric sensing lanthanides. 25199650 10.1021/ac5029962 RNA cleavage

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