DNAmoreDB - A Database of Deoxyribozymes

DNAzyme description

 Reaction  Reacting groups  Substrates Product  Metal ion Linkage  Seq description
RNA cleavage Group 1 - 2'-OH of rA

Group 2 - vicinal phosphate

S: *
specific cleavage at desired position Na+
RNA phosphodiester N 50
 Buffer conditions
50 mM Bis-Tris pH 7.0, 1 mM EDTA, 10 mM sodium citrate, 105 mM NaCl
 kcat/ kobs
kobs = 0.1 min-1
 Catalytic region of the DNAzyme
GTTCAGACTAATCATCACGTATAGGAAGTACCGCATGGTACCAGGTCAAAGGTGGGTGAGGGGACGCCAAGAGTCCCCGCGGTTACGTGATGTGAGCCTACGATGAGAC
Notes
This is the cis-cleaving version of the DNAzyme. Sequences have been annotated as reported. The cleavage site rA is in position 23 in the reported sequence. The trans-cleaving DNAzyme is annotated as NaA43E.

 Reported in...

Year of Publication First Author Laboratory Title PubMed ID DOI Reaction
2015 S-F Torabi Y Lu In vitro selection of a sodium-specific DNAzyme and its application in intracellular sensing None 10.1073/pnas.1420361112 RNA cleavage

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