DNAmoreDB - A Database of Deoxyribozymes

DNAzyme description

 Reaction  Reacting groups  Substrates Product  Metal ion Linkage  Seq description
RNA cleavage Group 1 - 2'-OH of rA

Group 2 - vicinal phosphate

S: ACTCACTATrAGGAAGAGATGGACGTG
specific cleavage at desired position UO22+
RNA phosphodiester N *
 Buffer conditions
50 mM MES pH 5.5, 250 mM NaNO3, 0.1 mM UO22+
 kcat/ kobs
kobs = 1 min-1
 Catalytic region of the DNAzyme
CACGTCCATCTCTGCAGTCGGGTAGTTAAACCGACCTTCAGACATAGTGAGT
Notes
Designed to act in trans from Clone39 After truncation and rational design of substrate binding sequences.

 Reported in...

Year of Publication First Author Laboratory Title PubMed ID DOI Reaction
2007 J Liu Y Lu A catalytic beacon sensor for uranium with parts-per-trillion sensitivity and millionfold selectivity. 17284609 10.1073/pnas.0607875104 RNA cleavage

 Related Publications

Year of Publication First Author Laboratory Title PubMed ID DOI Reaction
2009 A K Brown Y Lu Biochemical characterization of a uranyl ion-specific DNAzyme. 19142882 10.1002/cbic.200800632 RNA cleavage
2013 P Wu Y Lu A DNAzyme-gold nanoparticle probe for uranyl ion in living cells. 23531046 10.1021/ja400150v RNA cleavage
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