DNAmoreDB - A Database of Deoxyribozymes

DNAzyme description

 Reaction  Reacting groups  Substrates Product  Metal ion Linkage  Seq description
RNA cleavage Group 1 - 2'-OH of rA*

Group 2 - vicinal phosphate

S: cleavage in cis
specific cleavage at desired position Zn2+
RNA phosphodiester N 50
 Buffer conditions
25 mM NaCl, 50 mM MOPS pH 7.5, Zn2+
 Catalytic region of the DNAzyme
CTGCAGAATTCTAATACGAGTCACTATAGGAAGATGGCGAAACATTCTAGATGTAAGTAAATCTTCTCTCAGCGAGACGAAATAGTGACGAGGATAAGGGTGGTCGGTAAGCTTGGCAC
Notes
The cleavage junction is modified with a glycyl-histidine functionalized tertiary amine moiety. Sequences annotated directly after selection, this means that the sequences contain the catalytic region and also the constant regions (including the substrate).

 Reported in...

Year of Publication First Author Laboratory Title PubMed ID DOI Reaction
2020 P-J J Huang J Liu Target Self-Enhanced Selectivity in Metal-Specific DNAzymes. 31867832 10.1002/anie.201915675 RNA cleavage

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