DNAmoreDB - A Database of Deoxyribozymes

DNAzyme description

 Reaction  Reacting groups  Substrates Product  Metal ion Linkage  Seq description
RNA cleavage Group 1 - 2'-OH of rU

Group 2 - vicinal phosphate

S: GAGAGAGATrUCGTGCGTTAC
cleavage at UC junction Mn2+
RNA phosphodiester N 20
 Buffer conditions
100 mM KCl, 400 mM NaCl, 7.5 mM MgCl2 , 7.5 mM MnCl2, 50 mM HEPES pH 7.0
 Yield (%) kcat/ kobs
85 kobs = 0.009 min-1
 Catalytic region of the DNAzyme
CGGGGTTTGGGATGAAGGGG

 Reported in...

Year of Publication First Author Laboratory Title PubMed ID DOI Reaction
2008 K Schlosser Y Li In vitro selection of small RNA-cleaving deoxyribozymes that cleave pyrimidine-pyrimidine junctions. 18644842 10.1093/nar/gkn396 RNA cleavage

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