| Reaction | Reacting groups | Substrates | Product | Metal ion | Linkage | Seq description | RNA cleavage |
Group 1 - 2'-OH of rC Group 2 - vicinal phosphate |
S: cleavage in cis |
specific cleavage at desired position (C8) |
M2+-independent |
RNA phosphodiester | N 20 |
|---|
| Buffer conditions |
|---|
| 50 mM sodium cacodylate pH 7.4, 0.2 M NaCl, 1 mM EDTA |
| Yield (%) | kcat/ kobs |
|---|---|
| 60 | kcat = 0.0044 min-1 |
| Catalytic region of the DNAzyme |
|---|
| GCGTGCCCGTCTGTTGGGCCCTACCAACAGUUCUCAUCCGUAGUGAAGGCACGCCCCGCGAGCUC |
| Notes |
|---|
| DU<sup>aa</sup>TP and dA<sup>im</sup>TP are present in the DNAzyme's catalytic region to incorporate protein-like functionalities. The catalytic domain starts at position 31 of the reported construct and is 18-nt long. |
| Year of Publication | First Author | Laboratory | Title | PubMed ID | DOI | Reaction |
|---|---|---|---|---|---|---|
| 2001 | D M Perrin | C Hélène | Bridging the gap between proteins and nucleic acids: a metal-independent RNAseA mimic with two protein-like functionalities. | 11456753 | 10.1021/ja003290s | RNA cleavage |
| Year of Publication | First Author | Laboratory | Title | PubMed ID | DOI | Reaction |
|---|---|---|---|---|---|---|
| 2002 | L Lermer | D M Perrin | Toward an RNaseA mimic: A DNAzyme with imidazoles and cationic amines. | 12188639 | 10.1021/ja0205075 |
RNA cleavage |