| Reaction | Reacting groups | Substrates | Product | Metal ion | Linkage | Seq description | RNA cleavage |
Group 1 - 2'-OH of rC Group 2 - vicinal phosphate |
S: cleavage in cis |
specific cleavage at desired position |
M2+-independent |
RNA phosphodiester | N 40 |
|---|
| Buffer conditions |
|---|
| 51 mM sodium cacodylate pH 7.4, 200 mM NaCl, 1 mM EDTA |
| Yield (%) | kcat/ kobs |
|---|---|
| 80 | kobs = 0.57 min-1 |
| Catalytic region of the DNAzyme |
|---|
| GCGTGCCCGTCTGTTGGGCCCTACCAACACUAGCAGCGCAAGUGAGGCGCGCUAUGAGUGUGCGUGCGUGUAUGCACGCCCC |
| Notes |
|---|
| DA<sup>im</sup>TP-, dU<sup>ga</sup>TP- and dC<sup>aa</sup>TP--modified DNAzyme. Made to act in trans. |
| Year of Publication | First Author | Laboratory | Title | PubMed ID | DOI | Reaction |
|---|---|---|---|---|---|---|
| 2009 | M Hollenstein | D M Perrin | A DNAzyme with three protein-like functional groups: enhancing catalytic efficiency of M2+-independent RNA cleavage. | 19591189 | 10.1002/cbic.200900314 | RNA cleavage |