DNAmoreDB - A Database of Deoxyribozymes

DNAzyme description

 Reaction  Reacting groups  Substrates Product  Metal ion Linkage  Seq description
RNA ligation Group 1 - 2',3'-cyclic phosphate

Group 2 - 5'-OH

L: UAAUACGACUCACUAUA
R: GGAAGAGAUGGCGACGG
non-native RNA Mg2+
2',5' N 20
 Buffer conditions
50 mM HEPES pH 7.5, 150 mM NaCl, 2 mM KCl, 40mM MgCl2
 Yield (%) kcat/ kobs
30 kobs = 0.19 (h-1) at pH 7.5, kobs = 2.4 (h-1) at pH 9.0
 Catalytic region of the DNAzyme
TTACGTGGAGGTGGGCTCTA

 Reported in...

Year of Publication First Author Laboratory Title PubMed ID DOI Reaction
2003 A Flynn-Charlebois S K Silverman In vitro evolution of an RNA-cleaving DNA enzyme into an RNA ligase switches the selectivity from 3'-5' to 2'-5'. 12720447 10.1021/ja0340331 RNA ligation

 Related Publications

Year of Publication First Author Laboratory Title PubMed ID DOI Reaction
2003 B L Ricca S K Silverman Optimization and generality of a small deoxyribozyme that ligates RNA. 12860124 10.1016/S0022-2836(03)00654-5 RNA ligation
2004 T K Prior S K Silverman Structure-function correlations derived from faster variants of a RNA ligase deoxyribozyme. 14960718 10.1093/nar/gkh263 RNA ligation
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