DNAmoreDB - A Database of Deoxyribozymes

DNAzyme description

 Reaction  Reacting groups  Substrates Product  Metal ion Linkage  Seq description
RNA ligation Group 1 - 2',3'-cyclic phosphate

Group 2 - 5'-OH

L: UAAUACGACUCACUAUA
R: GGAAGAGAUGGCGACGG
non-native RNA Mg2+
2',5' N 40
 Buffer conditions
50 mM HEPES pH 7.5, 150 mM NaCl, 2 mM KCl, 40 mM MgCl2
 Yield (%) kcat/ kobs
40-60 kobs = 0.08 (h-1) at pH 7.5, kobs = 0.023 (min-1) at pH 9.0
 Catalytic region of the DNAzyme
TGCGTTCTGATGAATTGCACATAGCTTATAGTTCCTTGCT

 Reported in...

Year of Publication First Author Laboratory Title PubMed ID DOI Reaction
2003 A Flynn-Charlebois S K Silverman Deoxyribozymes with 2'-5' RNA ligase activity. 12603132 10.1021/ja028774y RNA ligation

 Related Publications

Year of Publication First Author Laboratory Title PubMed ID DOI Reaction
2004 T K Prior S K Silverman Structure-function correlations derived from faster variants of a RNA ligase deoxyribozyme. 14960718 10.1093/nar/gkh263 RNA ligation
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