DNAmoreDB - A Database of Deoxyribozymes

DNAzyme description

 Reaction  Reacting groups  Substrates Product  Metal ion Linkage  Seq description
RNA ligation Group 1 - internal 2'-OH

Group 2 - 5'-triphosphate

L: UAAUACGACUCACUAUX
R: GGAAGAGAUGGCGACGG
X: A, C, dA, dC
branched RNA Mn2+
Mg2+
U14 N 40
 Buffer conditions
50 mM HEPES pH 7.5, 20 mM MnCl2
 Yield (%) kcat/ kobs
>85 kobs = 0.81 (min-1) at pH 7.5
 Catalytic region of the DNAzyme
GGGATGTGGGGCGCCACCAAGTTAATGTTTGGTTTGGGGA
Notes
LARIAT FORMATION

 Reported in...

Year of Publication First Author Laboratory Title PubMed ID DOI Reaction
2003 Y Wang S K Silverman Deoxyribozymes that synthesize branched and lariat RNA. 12783536 10.1021/ja035150z RNA ligation

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