DNAmoreDB - A Database of Deoxyribozymes

DNAzyme description

 Reaction  Reacting groups  Substrates Product  Metal ion Linkage  Seq description
RNA ligation Group 1 - 2',3 '- diol

Group 2 - 5'-triphosphate

L: GGAAGUCUCAUGUACUA
R: GAUGUUCUAGCGCCGGA
native RNA Mn2+
Mg2+
3',5' N 40
 Buffer conditions
50 mM CHES pH 9.0, 150 mM NaCl, 2 mM KCl, 40 mM MgCl2
 Yield (%) kcat/ kobs
60-70 kobs = 0.016 (min-1) at pH 9.0
 Catalytic region of the DNAzyme
GGATCATACGGTCGGAGGGGTTTGCCGTTTA
3D structures
5CKI
5CKK

 Reported in...

Year of Publication First Author Laboratory Title PubMed ID DOI Reaction
2010 F Wachowius C Höbartner Combinatorial mutation interference analysis reveals functional nucleotides required for DNA catalysis. 20872387 10.1002/anie.201003940 RNA ligation

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