DNAmoreDB - A Database of Deoxyribozymes

DNAzyme description

 Reaction  Reacting groups  Substrates Product  Metal ion Linkage  Seq description
RNA ligation Group 1 - internal 2'-OH

Group 2 - 5'-triphosphate

L: GGAUAAUACGACUCACUGCG
R: GGAAGAGAUGGCGACGG
branched RNA Mn2+
A11 N *
 Buffer conditions
50 mM HEPES pH 7.5, 20 mM MnCl2, 150 mM NaCl, 2 mM KCl
 Catalytic region of the DNAzyme
CCGACGCGTCCAGGAGGCAAGGGCTATATGCACCGCAGTGAGGGCTCGG
Notes
Pool N33-CGCAGTGAG-N7

 Reported in...

Year of Publication First Author Laboratory Title PubMed ID DOI Reaction
2011 C S Lee S K Silverman Improved deoxyribozymes for synthesis of covalently branched DNA and RNA. 20739352 10.1093/nar/gkq753 RNA ligation

 Related Publications

Year of Publication First Author Laboratory Title PubMed ID DOI Reaction
2008 T P Mui S K Silverman Convergent and general one-step DNA-catalyzed synthesis of multiply branched DNA. 18808125 10.1021/ol801568q DNA ligation
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