DNAmoreDB - A Database of Deoxyribozymes

DNAzyme description

 Reaction  Reacting groups  Substrates Product  Metal ion Linkage  Seq description
RNA cleavage Group 1 - 2'-OH of rA

Group 2 - vicinal phosphate

S: ACTCACTATrAGGAAGAGATG
specific cleavage at desired position Zn2+
RNA phosphodiester N 40
 Buffer conditions
1 mM ZnCl2, 500 mM NaCl and 50 mM HEPES pH 7.0
 kcat/ kobs
kobs = 0.02 min-1
 Catalytic region of the DNAzyme
CAAAGATGCCAGCATGCTATTCTCCGAGCCGGTCGAAATA

 Reported in...

Year of Publication First Author Laboratory Title PubMed ID DOI Reaction
2000 J Li Y Lu In vitro selection and characterization of a highly efficient Zn(II)-dependent RNA-cleaving deoxyribozyme. 10606646 10.1093/nar/28.2.481 RNA cleavage

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