| Reaction | Reacting groups | Substrates | Product | Metal ion | Linkage | Seq description | RNA cleavage |
Group 1 - 2'-OH of A8 Group 2 - vicinal phosphate |
S: NNNNNNNAGNNNNNN |
specific cleavage at desired position |
Mg2+ Zn2+ Pb2+ Ca2+ |
RNA phosphodiester | N 50 |
|---|
| Buffer conditions |
|---|
| 2 mM MgCl2, 150 mM NaCl, and 50 mM Tris⋅HCl pH 7.5 |
| Catalytic region of the DNAzyme |
|---|
| TCCGAGCCGGACGA |
| Notes |
|---|
| Obtained after re-selection |
| 3D structures |
|---|
| 5XMA |
| 5XM9 |
| 5XM8 |
| Year of Publication | First Author | Laboratory | Title | PubMed ID | DOI | Reaction |
|---|---|---|---|---|---|---|
| 1997 | S W Santoro | G F Joyce | A general purpose RNA-cleaving DNA enzyme. | 9113977 | 10.1073/pnas.94.9.4262 | RNA cleavage |