DNAmoreDB - A Database of Deoxyribozymes

DNAzyme description

 Reaction  Reacting groups  Substrates Product  Metal ion Linkage  Seq description
RNA cleavage Group 1 - 2'-OH of A8

Group 2 - vicinal phosphate

S: NNNNNNNAGNNNNNN
specific cleavage at desired position Mg2+
Zn2+
Pb2+
Ca2+
RNA phosphodiester N 50
 Buffer conditions
2 mM MgCl2, 150 mM NaCl, and 50 mM Tris⋅HCl pH 7.5
 Catalytic region of the DNAzyme
TCCGAGCCGGACGA
Notes
Obtained after re-selection
3D structures
5XMA
5XM9
5XM8

 Reported in...

Year of Publication First Author Laboratory Title PubMed ID DOI Reaction
1997 S W Santoro G F Joyce A general purpose RNA-cleaving DNA enzyme. 9113977 10.1073/pnas.94.9.4262 RNA cleavage

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