DNAmoreDB - A Database of Deoxyribozymes

DNAzyme description

 Reaction  Reacting groups  Substrates Product  Metal ion Linkage  Seq description
RNA cleavage Group 1 - 2'-OH of R8

Group 2 - vicinal phosphate

S: NNNNNNNRYNNNNNN
specific cleavage at desired position Mg2+
RNA phosphodiester N 50
 Buffer conditions
2 mM MgCl2, 150 mM NaCl, and 50 mM Tris⋅HCl pH 7.5
 kcat/ kobs
kcat = 3.4 min-1
 Catalytic region of the DNAzyme
RGGCTAGCTACAACGA
Notes
Obtained after re-selection
3D structures
1BR3
1EGK

 Reported in...

Year of Publication First Author Laboratory Title PubMed ID DOI Reaction
1997 S W Santoro G F Joyce A general purpose RNA-cleaving DNA enzyme. 9113977 10.1073/pnas.94.9.4262 RNA cleavage

 Related Publications

Year of Publication First Author Laboratory Title PubMed ID DOI Reaction
2003 M J Cairns L‐Q Sun Optimisation of the 10-23 DNAzyme-substrate pairing interactions enhanced RNA cleavage activity at purine-cytosine target sites. 12771215 10.1093/nar/gkg378 RNA cleavage
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