DNAmoreDB - A Database of Deoxyribozymes

DNAzyme description

 Reaction  Substrates Product  Metal ion Linkage  Seq description
DNA cleavage S: GGATAATACGACTCACTATCGGAAGAGATGGCGACTTCG
hydrolyzed DNA Zn2+
DNA phosphodiester N 30
 Buffer conditions
70 mM HEPES pH 7.5, 1 mM ZnCl2, 150 mM NaCl
 Yield (%) kcat/ kobs
60-70 kobs = 0.19 h-1
 Catalytic region of the DNAzyme
GGATCATGGCGGTTTGGTTACGCTTTGCGT
Notes
Hydrolysis site TATC^GGAA, phosphate after hydrolysis on 5'

 Reported in...

Year of Publication First Author Laboratory Title PubMed ID DOI Reaction
2012 T E Velez S K Silverman Systematic evaluation of the dependence of deoxyribozyme catalysis on random region length. 23088677 10.1021/co300111f DNA cleavage

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