| Reaction | Reacting groups | Substrates | Product | Metal ion | Linkage | Seq description | RNA ligation |
Group 1 - internal 2'-OH Group 2 - 5'-triphosphate |
L: GGATAATACGXTTCACTGCG R: GGAAGAGAUGGCGACGG X: rA, rC |
branched RNA |
Mg2+ |
branch-site nucleotide X | N * |
|---|
| Buffer conditions |
|---|
| 50 mm CHES pH 9.0, 150 mM NaCl, 2 mM KCl, 40 mM MgCl2 |
| Yield (%) | kcat/ kobs |
|---|---|
| 61 | kobs = 0.0094 min-1 |
| Catalytic region of the DNAzyme |
|---|
| CAGCTATATGTGCTGGACTGAGAGGGGTAGTTTCGCAGTGAGGTGTAGG |
| Notes |
|---|
| Pool N33-CGCAGTGAG-N7, the deoxyribozyme's sequence is anotated with the 9-nt linker between the two initially randomized regions |
| Year of Publication | First Author | Laboratory | Title | PubMed ID | DOI | Reaction |
|---|---|---|---|---|---|---|
| 2008 | P I Pradeepkumar | Scott K Silverman | DNA-catalyzed formation of nucleopeptide linkages. | 18214866 | 10.1002/anie.200703676 | RNA ligation |