| Reaction | Reacting groups | Substrates | Product | Metal ion | Seq description | DNA phosphorylation |
Group 1 - ATP Group 2 - 5'-OH |
S: GGAAGAGTGGTGAC-N70-AGCTGATCCTGATGG |
5'-self phosphorylated DNA |
Mn2+ |
N 70 |
|---|
| Buffer conditions |
|---|
| 50 mM HEPES pH 7.0, 400 mM NaCl, 100 mM KCl, 5 mM MgCl2, 5 mM CaCl2, 5 mM MnCl2, 50 μM CuCl2, 1 mM ATP, 1 mM GTP |
| kcat/ kobs |
|---|
| kcat = 2.8 min-1 |
| Catalytic region of the DNAzyme |
|---|
| GTTGGTGATAAAGACCGCGCTAGGGTCTGCAAGGGAGGAGATAGAGTATTTAGGATCGTATGGGCGGTG |
| Year of Publication | First Author | Laboratory | Title | PubMed ID | DOI | Reaction |
|---|---|---|---|---|---|---|
| 2002 | W Wang | Y Li | Sequence diversity, metal specificity, and catalytic proficiency of metal-dependent phosphorylating DNA enzymes. | 11983339 | 10.1016/S1074-5521(02)00127-8 | DNA phosphorylation |
| Year of Publication | First Author | Laboratory | Title | PubMed ID | DOI | Reaction |
|---|---|---|---|---|---|---|
| 2005 | J C Achenbach | Y Li | Secondary-structure characterization of two proficient kinase deoxyribozymes. | 15751953 | 10.1021/bi0483054 |
DNA phosphorylation |