DNAmoreDB - A Database of Deoxyribozymes

DNAzyme description

 Reaction  Reacting groups  Substrates Product  Seq description
RNA cleavage Group 1 - 2'-OH

Group 2 - vicinal phosphate

X: F = fluorescein-dT, Q = DABCYL-dT
S: GATGTGTCCGTGCFrAQGGTTCGA
cleavage of single ribonucleotide phosphodiester N 70
 Buffer conditions
400 mM NaCl, 100 mM KCl, 8.5 mM MgCl2, 5 mM MnCl2, 1.25 mM CdCl2, 0.25 mM NiCl2, citrate pH 4.0
 Catalytic region of the DNAzyme
TGAATAGGGTCCTAGGCATAAATTACGAAAACGGCTTTAATCTTTTAGTGGAAAGGTCCGATAACGAGTG
Notes
Evolved from a population initially selected at pH 4.0. Also appeared in pH3, pH5 pools as pH3DZ10 and pH5DZ1

 Reported in...

Year of Publication First Author Laboratory Title PubMed ID DOI Reaction
2003 Z Liu Y Li Assemblage of signaling DNA enzymes with intriguing metal-ion specificities and pH dependences. 12812493 10.1021/ja035208+ RNA cleavage

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