DNAmoreDB - A Database of Deoxyribozymes

DNAzyme description

 Reaction  Substrates Product  Seq description
Porphyrin metalation S: protoporphyrin IX (PPIX)/ mesoporphyrin IX (MPIX)
Cu-MPIX N *
 Buffer conditions
100 mM Tris pH 7.1, 225 mM KOAc, 5% DMSO, 0.5% Triton X-100
 kcat/ kobs
kcat = 1.30 min-1
 Catalytic region of the DNAzyme
GTGGGTCATTGTGGGTGGGTGTGG
Notes
24-nt-long sequence from within PS5.ST1. The reported buffer conditions were used in assays to determine the optimal sequence (derived from PS5.ST1) for catalysis. Further analyses were carried out on this DNAzyme, and optimized reaction conditions are reported in the publication.

 Reported in...

Year of Publication First Author Laboratory Title PubMed ID DOI Reaction
1997 Y Li D Sen Toward an efficient DNAzyme. 9154943 10.1021/bi962694n Porphyrin metalation

 Related Publications

Year of Publication First Author Laboratory Title PubMed ID DOI Reaction
1996 Y Li D Sen A catalytic DNA for porphyrin metallation None 10.1038/nsb0996-743 Porphyrin metalation
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