DNAmoreDB - A Database of Deoxyribozymes

Published on 2013 in Proc. Natl. Acad. Sci. U.S.A. volume 110 issue 14.

PubMed ID: 23509279

DOI:10.1073/pnas.1221946110

Abstract:

Catalytic DNA sequences (deoxyribozymes, DNA enzymes, or DNAzymes) have been identified by in vitro selection for various catalytic activities. Expanding the limits of DNA catalysis is an important fundamental objective and may facilitate practical utility of catalysts that can be obtained from entirely unbiased (random) sequence populations. In this study, we show that DNA can catalyze Zn2+-dependent phosphomonoester hydrolysis of tyrosine and serine side chains (i.e., exhibit phosphatase activity). The best deoxyribozyme decreases the half-life for phosphoserine hydrolysis from as high as >1010 y to <1 h. The phosphatase activity also occurs with nonpeptidic substrates but with reduced efficiency, indicating a preference for phosphopeptides. The newly identified deoxyribozymes can function with multiple turnover using free peptide substrates, have activity in the presence of human cell lysate or BSA, and catalyze dephosphorylation of a larger protein substrate, suggesting broader application of DNA catalysts as artificial phosphatases.



DNAzymes linked to this article:

Name Isolated sequence Length Reaction
14WM9 CGCAGCGAAATCGGTCTTTATAGGGGCTGTCCTCCGACGG      40 Dephosphorylation
14WM13 GCCCGACGCGAATCCCCCCAGCTTGGGGCTGTCCACCGAC      40 Dephosphorylation
14WM16 CAAAACACGCGACCTTAACTAATGGGGCATGTCCTCCGAC      40 Dephosphorylation
14WM23 AGCCTGAAGGAGTTGCCTTCATGGGGGTGTTACTCCCAAT      40 Dephosphorylation
14WM27 GAGAAGCTGTCCTTGTCACTGGTGGGGCAGTCCTCCGACA      40 Dephosphorylation
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