DNAmoreDB - A Database of Deoxyribozymes

Published on 2020 in Angew. Chem. Int. Ed. Engl..

PubMed ID: 32681686

DOI:10.1002/anie.202006218

Abstract:

RNA‐cleaving deoxyribozymes can serve as selective sensors and catalysts to examine the modification state of RNA. However, site‐specific endonuclease deoxyribozymes that selectively cleave posttranscriptionally modified RNA are extremely rare and their specificity over unmodified RNA is low. In this study, we report that the native tRNA modification N 6 ‐isopentenyladenosine (i 6 A) strongly enhances the specificity and has the power to reconfigure the active site of an RNA‐cleaving deoxyribozyme. Using in vitro selection, we identified a DNA enzyme that cleaves i 6 A‐modified RNA at least 2500‐fold faster than unmodified RNA. Another deoxyribozyme shows unique and unprecedented behavior by shifting its cleavage site in the presence of the i 6 A RNA modification. Together with deoxyribozymes that are strongly inhibited by i 6 A, these results highlight intricate ways of modulating the catalytic activity of DNA by posttranscriptional RNA modifications.



DNAzymes linked to this article:

Name Isolated sequence Length Reaction
AA07 GGGTTCGGTGGAGCGGCGCGA      21 RNA cleavage
AA14 TGGTCTCGCGGTTCCTGGTTA      21 RNA cleavage
AA17 CCTGCAAGGAGGTTTACCGGG      21 RNA cleavage
AB08 GGGAAGCCAGTGGTACGTT      19 RNA cleavage
AC17 GGGTCTCCAGCCGGACGTTA      20 RNA cleavage
Copyright © Genesilico - All rights reserved
This website is free, open to all users and there is no login required.
If you have any advice or suggestions for corrections or improvements, please contact: Almudena Ponce Salvatierra