DNAmoreDB - A Database of Deoxyribozymes

Abstract:

An RNA ligase ribozyme was converted to a corresponding deoxyribozyme through in vitro evolution. The ribozyme was prepared as a DNA molecule of the same sequence, and had no detectable activity. A population of randomized variants of this DNA was constructed and evolved to perform RNA ligation at a rate similar to that of the starting ribozyme. When the deoxyribozyme was prepared as an RNA molecule of the same sequence, it had no detectable activity. Thus, the evolutionary transition from an RNA to a DNA enzyme represents a switch, rather than a broadening, of the chemical basis for catalytic function. This transfer of both information and function is relevant to the transition between two different genetic systems based on nucleic acid-like molecules, as postulated to have occurred during the early history of life on Earth.

DNAzymes linked to this article:

Name	Isolated sequence	Length	Reaction
10-13	CTAACCACTATCCATGATTGTAAGAGCGGACAGCTGCAACGGGTTGATTATAGTGGGG	58	RNA ligation
10-9	CTAACCATTATCCTCGATTGTTAGAACGAACAGTTGCAACGGGTTGATTATAGTGGG	57	RNA ligation
10-19	TAACCCATTATCTTCGATGTTAGAACGAACAGCTGCAACGGGTTGATTATAGTGAG	56	RNA ligation
10-15	CTCGCCATTATCCTTGAGTGTTAGAACGAACAGTTGCAACGGGTTGATTATAGTGAG	57	RNA ligation
10-7	CTATGCATTATCCTTGACTGTTAGATCGAGCAGTTGCAACGGGTTGATTATAGTGAG	57	RNA ligation
10-22	CTAACCATTATCCCTGATTGTTAGAACGAACAGTTGCGACGGGTTGATTATAGTGAC	57	RNA ligation
10-6	CTACGCATTATCCCTGGTTGTTAGAACGAGCAGTTGCAACGGGTTGATTATAGTGAC	57	RNA ligation
10-21	CAAACCATTATCCTCGATTGCAAGAACGAGCAGTTGGAACGGGTTGATTGTAGTGAGG	58	RNA ligation
10-3	CATATTCATCATCCTCGACTGCAAGAAAGAACAGTTGGAACGGGTTGAATATAGTGAG	58	RNA ligation
10-5	CTCCTCATTATTCACGAATGATAGCACGAATAGTGTGAACGGGTTGATTATAGTGGCG	58	RNA ligation
10-17	CTCCTCATTATTCTAGAATGATAGCACGAATAGTGTGAACGGGTTGATTATAGTGGCG	58	RNA ligation
10-4	CAGCTCATTATTCACGAATGATGCACGAATAGTGTGAACGGGTTGATTATAGTGGG	56	RNA ligation
10-12	AACCCTCATTATCCACCAATGATAGCACGAATAGTGTGAGCGGGTTGATTATAGTGAG	58	RNA ligation
10-14	AGGACTCATTAAGCTCGATTGCTCGAACGAACGGTTGGCACGGGTTGATTATAGTG	56	RNA ligation
10-8	CGCAGCATTATGCTCGCATGCCCGAACGAACGGTTGGCACGGGTTGATTATAGTGGG	57	RNA ligation
10-2	CGACTGGTTTTGCTCGTTTCTTCGTAGGAACAGATGTAATGGGTTGATATAGTGGCG	57	RNA ligation
10-10	CGACTGGCTCTGCTCGTTTCTTCGAATGAACAGATGTAGTGGGTTGATATAGCGCG	56	RNA ligation
10-16	CGACTAATCATGCTCGAATGTTCGTAAGAACAGTCTGTGCGGGTGGAATATAGTGAG	57	RNA ligation
10-1	ACTGACTCATACTGCACGCTTGTCCCTAAGGTAGTTGCGCAGGTGGAATATAGTGGG	57	RNA ligation
10-11	CTACGGGTTATGGTTGATTATACGTAAAAACAGTTAGGATGAGTTGTTGGTCGTGG	56	RNA ligation
10-18	CTAACCATTATCCTCGATTGTTAGAGCGAACAGCTGCAACGGGTTGATTATAGTGAG	57	RNA ligation
10-20	CTACCCATTATTCACGAATGATAGCACGAATAGTGTGAACGGGTTGATTATAGTGAC	57	RNA ligation

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