DNAmoreDB - A Database of Deoxyribozymes

DNAzyme description

 Reaction  Reacting groups  Substrates Product  Metal ion Linkage  Seq description
Tyrosine azido‐adenylylation Group 1 - Tyrosine's hydroxyl

Group 2 - 2′‐Az‐dATP

L: DNA-anchored CAAYAA hexapeptide
R: 2′‐Az‐dATP
Azido-adenylated peptide Mn2+
Mg2+
Zn2+
phosphodiester N 40
 Buffer conditions
70 mm HEPES pH 7.5, 1 mm ZnCl2, 20 mm MnCl2, 40 mm MgCl2, 150 mm NaCl
 Yield (%) kcat/ kobs
56 kobs = 0.19 h-1
 Catalytic region of the DNAzyme
AAGCACCAATGCGATGGACGGGGGCGGGCCACCGTGACAC
Notes
Reselected starting from DzAz1

 Reported in...

Year of Publication First Author Laboratory Title PubMed ID DOI Reaction
2016 P Wang S K Silverman DNA-Catalyzed Introduction of Azide at Tyrosine for Peptide Modification. 27391404 10.1002/anie.201604364 Tyrosine azido‐adenylylation

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