DNAmoreDB - A Database of Deoxyribozymes

Published on 2016 in Angew. Chem. Int. Ed. Engl. volume 55 issue 34.

PubMed ID: 27391404

DOI:10.1002/anie.201604364

Abstract:

We show that DNA enzymes (deoxyribozymes) can introduce azide functional groups at tyrosine residues in peptide substrates. Using in vitro selection, we identified deoxyribozymes that transfer the 2′‐azido‐2′‐deoxyadenosine 5′‐monophosphoryl group (2′‐Az‐dAMP) from the analogous 5′‐triphosphate (2′‐Az‐dATP) onto the tyrosine hydroxyl group of a peptide, which is either tethered to a DNA anchor or free. Some of the new deoxyribozymes are general with regard to the amino acid residues surrounding the tyrosine, while other DNA enzymes are sequence‐selective. We use one of the new deoxyribozymes to modify free peptide substrates by attaching PEG moieties and fluorescent labels.



DNAzymes linked to this article:

Name Isolated sequence Length Reaction
DzAz1b ACCCTTCACAGCAAACAAGGGGGGCGGGCCACCGTGACCC      40 Tyrosine azido‐adenylylation
DzAz1c AAGCACCAATGCGATGGACGGGGGCGGGCCACCGTGACAC      40 Tyrosine azido‐adenylylation
DzAz2 GCACATACCGAATCAGAGCGGTGGCCAGGACATGAGATAA      40 Tyrosine azido‐adenylylation
DzAz3 CCAACATAGGATGAGGAAGGGTAGGGACTTCCCGTGACTG      40 Tyrosine azido‐adenylylation
DzAz4 CGAACACACGTGGAGGTCGAGTTCGAATGTTTGATCGGTA      40 Tyrosine azido‐adenylylation
DzAz5 GATGCGACTCGTCGTACGTAGGGGTAGGGATCCCCGTGAC      40 Tyrosine azido‐adenylylation
DzAz6 GTGTCCTAGTAAGAGTGATGGGGGTAGGGACCCCCGCGAC      40 Tyrosine azido‐adenylylation
DzAz7 GCATACTGTTAGGGCTACAGATATACGTATATCTGCGCTA      40 Tyrosine azido‐adenylylation
DzAz8 ATCGTCTCTAACTCTGGGGGCATAGGGCTGCCCGTGACTA      40 Tyrosine azido‐adenylylation
DzAz9 AGCCCCTTACGCAGAAATAGAGAGGGGCGGGTCCCGTGAC      40 Tyrosine azido‐adenylylation
DzAz1 AACCACCTTTGTATAGTTGGGGGGCGGGCCACCGTGACAC      40 Tyrosine azido‐adenylylation
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