DNAmoreDB - A Database of Deoxyribozymes

DNAzyme description

 Reaction  Substrates  Metal ion  Seq description
DNA cleavage S: GGATAATACGACTCACTATTTGAAGAGATGGCGACTTCG
Mg2+
Zn2+
N 100
 Buffer conditions
70 mM HEPES pH 7.5, 1 mM ZnCl2, 20 mM MnCl2, 40 mM MgCl2, 150 mM NaCl
 Yield (%) kcat/ kobs
40 kobs = 1 h-1
 Catalytic region of the DNAzyme
TAAGATAAACACACCGGCCGTCACGCTGCCTCAGACGGTGAGTGACCCTAAAATAAGGGGATAACCCAAGGCCGATATCCCAATTTGAGATGGTCGGGGA
Notes
This DNAzyme catalyzes a radical-based reaction pathway that results in excision of most atoms of a specific guanosine nucleoside.

 Reported in...

Year of Publication First Author Laboratory Title PubMed ID DOI Reaction
2017 Y Lee S K Silverman DNA-Catalyzed DNA Cleavage by a Radical Pathway with Well-Defined Products. 27935689 10.1021/jacs.6b10274 DNA cleavage

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