DNAmoreDB - A Database of Deoxyribozymes

DNAzyme description

 Reaction  Reacting groups  Substrates Product  Metal ion Linkage  Seq description
RNA cleavage Group 1 - 2'-OH of rA

Group 2 - vicinal phosphate

S: GTCACGAGTCACTATrAGGAAGATGGCGAAA
specific cleavage at desired position Na+
M2+-independent
RNA phosphodiester N 50
 Buffer conditions
50 mM MES pH 6.0, 130 mM NaCl, 60% (V/V) alcohol
 kcat/ kobs
kobs = 2 h-1
 Catalytic region of the DNAzyme
TTTCGCCATCTTTTCTCACACCGTACTCGGTAAGGTTGTTAGTGACTCGTGAC
Notes
Discovered accidentally. The in vitro selection was intended for the isolation of hemin-dependent RNA-cleaving DNAzymes. Of the 34 isolated sequences from the in vitro selection, 30 were identical. One of them was engineered into the trans-cleaving DNAzyme EtNa.

 Reported in...

Year of Publication First Author Laboratory Title PubMed ID DOI Reaction
2015 W Zhou J Liu A New Na+‐Dependent RNA‐Cleaving DNAzyme with over 1000‐fold Rate Acceleration by Ethanol None 10.1002/cbic.201500603 RNA cleavage

 Related Publications

Year of Publication First Author Laboratory Title PubMed ID DOI Reaction
2017 W Zhou J Liu Two Completely Different Mechanisms for Highly Specific Na+ Recognition by DNAzymes None 10.1002/cbic.201700184 RNA cleavage
2017 W Zhou J Liu An Exceptionally Selective DNA Cooperatively Binding Two Ca Ions. 28087991 10.1002/cbic.201600708 RNA cleavage
2018 T Yu J Liu An RNA-Cleaving Catalytic DNA Accelerated by Freezing. 29537685 10.1002/cbic.201800049 RNA cleavage
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