DNAmoreDB - A Database of Deoxyribozymes

DNAzyme description

 Reaction  Reacting groups  Substrates Product  Metal ion Linkage  Seq description
RNA cleavage Group 1 - 2'-OH of rA

Group 2 - vicinal phosphate

S: cleavage in cis
specific cleavage at desired position Co2+
RNA phosphodiester N 40
 Buffer conditions
50 mM HEPES pH 7.0, 500 mM NaCl, 1 mM CoCl2
 kcat/ kobs
kobs = 0.175 min-1
 Catalytic region of the DNAzyme
TTGTATTAGCTACACTGTTAGTGCATCGTTTTTAATCTCG

 Reported in...

Year of Publication First Author Laboratory Title PubMed ID DOI Reaction
2002 P J Bruesehoff Y Lu Improving metal ion specificity during in vitro selection of catalytic DNA. 12052183 10.2174/1386207023330264 RNA cleavage

 Related Publications

Year of Publication First Author Laboratory Title PubMed ID DOI Reaction
2012 K E Nelson Y Lu The importance of peripheral sequences in determining the metal selectivity of an in vitro-selected Co(2+) -dependent DNAzyme. 22250000 10.1002/cbic.201100724 RNA cleavage
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