| Reaction | Reacting groups | Substrates | Product | Metal ion | Linkage | Seq description | RNA cleavage |
Group 1 - 2'-OH of rC Group 2 - vicinal phosphate |
S: GATGTGTCCGTGCTrCTGGTTCGATTTGTTT |
specific cleavage at desired position |
Mn2+ Mg2+ |
RNA phosphodiester | N 40 |
|---|
| Buffer conditions |
|---|
| 100 mM KCl, 400 mM NaCl, 50 mM Hepes pH 7.0 at 23 °C, 7.5 mM MgCl2, 7.5 mM MnCl2 |
| Yield (%) | kcat/ kobs |
|---|---|
| 60 | kobs = 0.34 min-1* |
| Catalytic region of the DNAzyme |
|---|
| CAAATTGATCGGTGGGGAGCAACTGAAAGGCGGTTGCAATGCGGATGGATGGTACGGTC |
| Notes |
|---|
| Made to act in trans from CT10-3.29. Extensive structure probing data. Mutants of this DNAzyme achieve greater cleavage rates and higher yields, i.e. CT10.3.29M1 has k<sub>obs</sub> = 1.4 min<sup>-1</sup>. |
| Year of Publication | First Author | Laboratory | Title | PubMed ID | DOI | Reaction |
|---|---|---|---|---|---|---|
| 2010 | J C F Lam | Y Li | A complex RNA-cleaving DNAzyme that can efficiently cleave a pyrimidine-pyrimidine junction. | 20630470 | 10.1016/j.jmb.2010.05.047 | RNA cleavage |