DNAmoreDB - A Database of Deoxyribozymes

DNAzyme description

 Reaction  Reacting groups  Substrates Product  Metal ion Linkage  Seq description
RNA ligation Group 1 - internal 2'-OH

Group 2 - 5'-triphosphate

L: UAAUACGACUCACUAUA
R: GGAAGAGAUGGCGACGG
branched RNA Mg2+
A8 N 37
 Buffer conditions
50 mM CHES pH 9.0, 150 mM NaCl, 2 mM KCl, 40 mM MgCl2
 Yield (%) kcat/ kobs
>90 kobs = 0.5 min-1
 Catalytic region of the DNAzyme
CAGTGCAGGGCGTGAGGGCTCGGTTCCCGTATTATCT
Notes
LARIAT FORMATION

 Reported in...

Year of Publication First Author Laboratory Title PubMed ID DOI Reaction
2004 R L Coppins S K Silverman A DNA enzyme that mimics the first step of RNA splicing. 14758353 10.1038/nsmb727 RNA ligation

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