DNAmoreDB - A Database of Deoxyribozymes

DNAzyme description

 Reaction  Reacting groups  Substrates  Metal ion  Seq description
Reductive amination Group 1 - Aldehyde on the capture oligonucleotide

Group 2 - N2-amine of the guanosine nucleobase in position 1 of the RNA substrate

L: NaIO4-oxidized capture oligonucleotide
R: 5'-mono or triphosphate RNA substrate complementary to the DNAzyme arm
Ni2+
NaCNBH3
NaIO4
N 40
 Buffer conditions
50 mM HEPES pH 7.5, 40 mM MgCl2, 20 mM MnCl2, 150 mM NaCl, 2 mM KCl
 Catalytic region of the DNAzyme
AAGGGGTAGTGCCAAACGTACGAAGGCTTCATTACCGACC
Notes
Not studied in detail. This selection originally intended to isolate DNAzymes capable of modifying free peptide substrates.

 Reported in...

Year of Publication First Author Laboratory Title PubMed ID DOI Reaction
2011 O Y Wong S K Silverman DNA-catalyzed reductive amination. 21994131 10.1002/anie.201104976 Reductive amination

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