DNAmoreDB - A Database of Deoxyribozymes

Published on 2013 in Anal. Chem. volume 85 issue 3.

PubMed ID: 23234289

DOI:10.1021/ac302721j

Abstract:

Capillary electrophoresis–systematic evolution of ligands by exponential enrichment (CE–SELEX) has previously been used to select aptamers for large-molecule targets such as proteins, lipopolysaccharides, and peptides. For the first time, we have performed CE–SELEX selection for a small-molecule target, N-methyl mesoporphyrin (NMM), with a molecular weight of only 580 g/mol. DNA aptamers with high-nanomolar to low-micromolar dissociation constants were achieved after only three rounds of selection. This corresponds to an >50-fold improvement in affinity over the random library. Two out of eight randomly chosen aptamers were found to catalyze the metal insertion reaction of mesoporphyrin with 1.7- and 2.0-fold rate enhancements, respectively.



DNAzymes linked to this article:

Name Isolated sequence Length Reaction
clone 3.5 AAAAGAATGCTCATCCTGGGGTAGGCATGAAGCGGGGCCG      40 Porphyrin metalation
clone 6.23 TGCTCGACTATTGAGTCAGGGGGTTGGGGCAACAATAAGC      40 Porphyrin metalation
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